H2N2V1, Main, Exploration, bibRecord, 002E35

[Comparative study of the biological properties of influenza virus preparations purified by centrifugation and chromatography].

Identifieur interne : 002E35 ( Main/Exploration ); précédent : 002E34; suivant : 002E36

[Comparative study of the biological properties of influenza virus preparations purified by centrifugation and chromatography].

Auteurs : A G Iordan ; L A Mazhul' ; O N Shcheglovitova ; O N Ageeva

Source :

Voprosy virusologii [ 0507-4088 ]

RBID : pubmed:6868552

Descripteurs français

KwdFr :
- Animaux, Centrifugation isopycnique (), Chromatographie sur DEAE-cellulose (), Chromatographie sur gel, Embryon de poulet, Spécificité d'espèce, Ultracentrifugation (), Virus de la grippe A (isolement et purification).
MESH :
- isolement et purification : Virus de la grippe A.
- Animaux, Centrifugation isopycnique, Chromatographie sur DEAE-cellulose, Chromatographie sur gel, Embryon de poulet, Spécificité d'espèce, Ultracentrifugation.

English descriptors

KwdEn :
- Animals, Centrifugation, Isopycnic (methods), Chick Embryo, Chromatography, DEAE-Cellulose (methods), Chromatography, Gel, Influenza A virus (isolation & purification), Species Specificity, Ultracentrifugation (methods).
MESH :
- isolation & purification : Influenza A virus.
- methods : Centrifugation, Isopycnic, Chromatography, DEAE-Cellulose, Ultracentrifugation.
- Animals, Chick Embryo, Chromatography, Gel, Species Specificity.

Abstract

A comparative study of chromatography and centrifugation as applied to purification of 2 influenza virus strains with different antigenic structure (H0N1 and H2N2) and of some biological properties of the resulting preparations was carried out. Certain strain-specific differences manifested in their chromatographic behaviour and in the degree of purification of virions from the allantoic fluid proteins were found. Some quantitative differences observed in the capacity of the resulting virus preparations to infect chick embryos and primary and continuous cells are insignificant and cannot be used for judgement of advantages of one or the other of the methods used for influenza virus purification.

PubMed: 6868552

Affiliations:

Le document en format XML

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<author><name sortKey="Mazhul, L A" sort="Mazhul, L A" uniqKey="Mazhul L" first="L A" last="Mazhul'">L A Mazhul'</name>
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<author><name sortKey="Shcheglovitova, O N" sort="Shcheglovitova, O N" uniqKey="Shcheglovitova O" first="O N" last="Shcheglovitova">O N Shcheglovitova</name>
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<author><name sortKey="Ageeva, O N" sort="Ageeva, O N" uniqKey="Ageeva O" first="O N" last="Ageeva">O N Ageeva</name>
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<date when="????"><PubDate><MedlineDate>1983 Mar-Apr</MedlineDate>
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<term>Chromatography, DEAE-Cellulose (methods)</term>
<term>Chromatography, Gel</term>
<term>Influenza A virus (isolation & purification)</term>
<term>Species Specificity</term>
<term>Ultracentrifugation (methods)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Centrifugation isopycnique ()</term>
<term>Chromatographie sur DEAE-cellulose ()</term>
<term>Chromatographie sur gel</term>
<term>Embryon de poulet</term>
<term>Spécificité d'espèce</term>
<term>Ultracentrifugation ()</term>
<term>Virus de la grippe A (isolement et purification)</term>
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<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A virus</term>
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<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus de la grippe A</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Centrifugation, Isopycnic</term>
<term>Chromatography, DEAE-Cellulose</term>
<term>Ultracentrifugation</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chick Embryo</term>
<term>Chromatography, Gel</term>
<term>Species Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Centrifugation isopycnique</term>
<term>Chromatographie sur DEAE-cellulose</term>
<term>Chromatographie sur gel</term>
<term>Embryon de poulet</term>
<term>Spécificité d'espèce</term>
<term>Ultracentrifugation</term>
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<front><div type="abstract" xml:lang="en">A comparative study of chromatography and centrifugation as applied to purification of 2 influenza virus strains with different antigenic structure (H0N1 and H2N2) and of some biological properties of the resulting preparations was carried out. Certain strain-specific differences manifested in their chromatographic behaviour and in the degree of purification of virions from the allantoic fluid proteins were found. Some quantitative differences observed in the capacity of the resulting virus preparations to infect chick embryos and primary and continuous cells are insignificant and cannot be used for judgement of advantages of one or the other of the methods used for influenza virus purification.</div>
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<name sortKey="Mazhul, L A" sort="Mazhul, L A" uniqKey="Mazhul L" first="L A" last="Mazhul'">L A Mazhul'</name>
<name sortKey="Shcheglovitova, O N" sort="Shcheglovitova, O N" uniqKey="Shcheglovitova O" first="O N" last="Shcheglovitova">O N Shcheglovitova</name>
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[Comparative study of the biological properties of influenza virus preparations purified by centrifugation and chromatography].

[Comparative study of the biological properties of influenza virus preparations purified by centrifugation and chromatography].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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